Olson , Howard Ceri , Douglas W. Morck , Andre G. Buret , and Ronald R. This article has been cited by other articles in PMC. Abstract The Calgary Biofilm Device CBD was used to form bacterial biofilms of selected veterinary gram-negative and gram-positive pathogenic bacteria from cattle, sheep, pigs, chicken, and turkeys.
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Introduction Historically, we have studied these microorganisms by culturing them in highly enriched liquid or solid media that artificially selects for less hardy bacteria 1 , 2 , 3. Materials and methods Organisms Bacterial isolates were obtained from clinical cases of infections of cattle, sheep, pigs, chickens and turkeys. Selection of antibiotics The antibiotics evaluated were those commonly approved for the treatment of bacterial infections in animals.
Minimum biofilm eradication concentration assay Assays were performed when pegs contained approximately 10 4 to 10 6 bacteria growing as a biofilm following conditions developed from the procedure described above. Determination of minimum inhibitory concentration The minimum inhibitory concentration MIC , which represents the concentration of antibiotic required to inhibit growth of a planktonic bacterial population, was determined using the CBD.
Open in a separate window. Discussion The MIC has been used as a gold standard for determination of antimicrobial sensitivities for animal and human pathogenic bacteria 4 , 5. Accepted November 29, References 1. Bacterial biofilms: a common cause of persistent infections.
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Human colon mucosal biofilms from healthy or colon cancer hosts are carcinogenic
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Protein Interactions. Protein Families. Nucleotide Sequences. Functional Genomics Experiments. Bacterial Infections.
Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention
Quorum Sensing. Signal Transduction. Sciences de la vie. Infectious Diseases. Cell Biology. Balaban, Naomi. Related item. Print version:: Control of biofilm infections by signal manipulation. Bibliography Illustrated text. Holdings information at the University of Warwick Library Requesting live circulation data Live circulation data is not available.
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Citations Publications citing this paper. The difference between native septic arthritis and prosthetic joint infections: A review of literature. Disinfection by-product formation from biofilm chlorination in drinking water pipes Carolina Montoya-Pachongo. Biotimer assay: A reliable and rapid method for the evaluation of central venous catheter microbial colonization.
The synergistic effects of orthogonal biofilm mitigation strategies: thermal and antibiotic treatment Erica B Ricker.
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